Evaluation of Peptide's property of binding to PMMA stereo- complex membrane

Evaluation of Peptide's property of binding to PMMA stereo- complex membrane

Evaluation of peptide's property of specifically binding to stereo complex Polymethacrylate (PMMA) and the evaluation of streptavidin's binding property via biotinylated peptide.

(Data provided by: Research Center for Advanced Science and Technology, University of Tokyo)

1.Evaluation of peptide binding

  • Sensor：30MHz　twin sensor
  • Flow rate：50μL/min
  • Sample amount：100μL
  • Running buffer：PBS
  • Membrane：stereocomplex, st-PMMA
  • Peptide：400μM, 100μL, 5min　MW＝855

Fig.1: Diagram

2.Result

Fig.2: Reaction waveform

Fig.3: Binding amount to stereo- complex membrane

3.Binding of streptavidin via biotinated peptide

  • Sensor：30MHz　twin sensor
  • Flow rate：50μL/min
  • Sample amount：100μL
  • Membrane：stereocomplex membrane
  • Peptide：Biotinylated peptide　MW＝1,247　400μM, 100μL, 5min
  • SAv（streptavidin）：MW＝53,000　250nM, 100μL

Fig.4: Diagram of biotinylated peptide

4.Measurement wave form

Fig.5: Reaction waveform

5.Glossary

PMMA (Poly Methyl Methacrylate) PMMA, poly methyl methacrylate, is a kind of acrylic resin
Stereocomplex membrane Spiral structure membrane in which 2 kinds of PMMA (isotactic and syndiotactic structure ) interact each other.
Avidin Glycoprotein contained in egg albumen. The molecular weight is approximately 67,000. The bond between avidin and biotin is very strong. Bacteria-derived Streptavidin (MW:approx.60,000) is a similar protein, and is often used as well. Since Streptavidin does not have sugar chain, less nonspecific binding occurs as compared with avidin.
Biotin A kind of water soluble vitamin that binds to avidin strongly. Molecular weight is approx. 244.
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