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Evaluation of Peptide's property of binding to PMMA stereo- complex membrane
Evaluation of peptide's property of specifically binding to stereo complex Polymethacrylate (PMMA) and the evaluation of streptavidin's binding property via biotinylated peptide.
(Data provided by: Research Center for Advanced Science and Technology, University of Tokyo)
1.Evaluation of peptide binding
- Sensor：30MHz twin sensor
- Flow rate：50μL/min
- Sample amount：100μL
- Running buffer：PBS
- Membrane：stereocomplex、st-PMMA
- Peptide：400μM, 100μL, 5min MW＝855
Fig.1: Diagram/p>
2.Result
Fig.2: Reaction waveform
Fig.3: Binding amount to stereo- complex membrane
3.Binding of streptavidin via biotinated peptide
- Sensor：30MHz twin sensor
- Flow rate：50μL/min
- Sample amount：100μL
- Membrane：stereocomplex membrane
- Peptide：Biotinylated peptide MW＝1,247 400μM, 100μL, 5min
- SAv（streptavidin）：MW＝53,000 250nM, 100μL
Fig.4: Diagram of biotinylated peptide
4.Measurement wave form
Fig.5: Reaction waveform
5.Glossary
| PMMA (Poly Methyl Methacrylate) | PMMA, poly methyl methacrylate, is a kind of acrylic resin |
| Stereocomplex membrane | Spiral structure membrane in which 2 kinds of PMMA (isotactic and syndiotactic structure ) interact each other. |
| Avidin | Glycoprotein contained in egg albumen. The molecular weight is approximately 67,000. The bond between avidin and biotin is very strong. Bacteria-derived Streptavidin (MW:approx.60,000) is a similar protein, and is often used as well. Since Streptavidin does not have sugar chain, less nonspecific binding occurs as compared with avidin. |
| Biotin | A kind of water soluble vitamin that binds to avidin strongly. Molecular weight is approx. 244. |
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